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Mosquito guts were dissected on microscopic slides in several drops of the solution, mounted on other microscopic slides in a drop of the staining solution and covered with cover slips.
The residual subsamples were mounted onto microscopic slides in Canada Balsam medium for photomicrography and in liquid medium for counting, measuring, and line drawing.
After a final rehydration step in PBS the animals were mounted on microscopic slides in PBS 90% glycerol.
Rehydration and washing were carried out by placing the microscopic slides in glass jars containing approximately 60 ml of the appropriate solutions.
Nonmigrated cells were scraped off using cotton wool tips, and membranes were recovered and mounted onto microscopic slides in VECTASHIELD HardSet mounting medium with DAPI (Vector laboratories).
Fifth, there was a variable number of patient samples in different immunohistochemical analyses, because the second best endomyocardial biopsy sample designated for immunohistochemical stainings was not sufficient for all microscopic slides in every patient (see online supplementary information).
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The roots were mounted on a microscopic slide in 50% glycerol in 1X PBS; 0.3-μm thick longitudinal optical sections were acquired on a Zeiss LSM-510 Meta confocal microscope using 63X oil immersion (NA 1.4) and were further projected to obtain the image of 2 to 3 μm total thickness.
Light extinction measurements were carried out using a compact localized surface plasmon resonance spectrometer 'NanoPLASMON-003' (NanoPlasmon Devices, Elmhurst, IL, USA) possessing a spectral range of 400 to 1,100 nm and compatible with nanochips of various size (within the limits of a 1 × 3 inch standard plain microscopic slide) in both stationary and flow real-time operation modes.
After washing (4 × 20 min) with PBS-T, larvae were washed in PBS and mounted on a microscopic slide in 80% (v/v) glycerol for microscopic examination.
The sections were mounted on Super Frost microscopic slides, washed in phosphate buffer, then immersed for 25 min in a dilute cresyl violet stain (0.01%) in acetate buffer (pH 4.5), dehydrated in serial dilutions of ethyl alcohol and finally coverslipped using DePeX (Serva, Heidelberg, Germany).
Performing duties relating to the administrative maintenance of surgical pathology protocols, reports and data, including the filing of reports, protocols, photographic data or slide, microscopic slides assisting in the completion of specimen coding and billing.
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