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On the 5th day (D4), thin blood films were made from the tail of each mouse on a microscopic slide (Citoplus, China).
At 10 days of age the flies were decapitated and the heads were mounted on a microscopic slide with nail polish.
Microscopic slide evaluation/image acquisition was performed using a Leica DM6000B light microscope (Leica, Wetzlar, Germany) and the Diskus Mikroskopische Diskussion image acquisition software (Carl H. Hilgers, Königswinter, Germany).
After exposure, Daphnia were placed on a microscopic slide and we examined the complete Daphnia body under a fluorescent microscope.
Collected stages III and IV larvae were placed individually in a depression microscopic slide with a minimum amount of water and identified under a light microscope with an objective (×10) using standard morphological keys developed for Sri Lankan anopheline larvae [ 17].
Sampling variables were at the mouse and microscopic slide and field levels.
We were stunned by, on any microscopic slide, literally hundreds of unique microorganisms swimming around at high speed.
A retrospective study was carried out with hospital records and a microscopic slide review from a large dermatology department in Mexico City, Mexico.
The standard microscopic slide format facilitates data capture with most automated array readers and, thus high sample throughput (>350 sample/h).
We thank the primary study participants and their parents, and village and district authorities, for their assistance obtaining samples in Mbita; Geoffrey Omondi Olweru and David John Odoyo for their contributions to the Mbita field work; Angela Hunt Cooke (MRL, LSHTM) for microscopic slide reading and Ernest Diez-Benavente (LSHTM) for helping with the access to the genomic data.
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The cells on the lower surface of the insert were stained with crystal violet and each transwell membrane mounted on a microscopic slide for visualization and analysis.
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