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There was a significant difference by means of both macroscopic and microscopic scores between group 2 (The colitis group) and other four groups (P < 0.05).
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Table 1 shows the macroscopic and microscopic scores of all rats involved in this study.
B. Microscopic scores were scored by a gastroenterological pathologist with experience in murine mucosal pathology (D Owen).
Data represents the median values (and range) of microscopic scores in at least 10 animals per group.
We demonstrate a correlation between oxidative stress, inflammation and angiogenesis, where increase in tpO2 and reduce oxidative stress observed in responders is associated with lower microscopic scores of T-cells (CD4 and CD8), B-cells (CD20), macrophages (CD68) and angiogenesis (VEGF).
Before reliable correlations can be made between macro and microscopic scoring systems these parameters need to be studied more extensively.
The concordance between both pathologists and the other two methods of scoring are between these two extremes, but the concordance is greater between the pathologist and the proposed automated method than between the pathologists and the classical microscopic score.
Microscopic scoring was performed manually with an Olympus BX60 fluorescence microscope with an Olympus × 100/1.30 oil lens.
Macro- and microscopic scoring was undertaken after three months.
Microscopic scoring was performed on coded slides [see also Supplemental Material (doi 10.1289/ehp.1001959)].
The results observed for serum liver enzymes (ALT, ALP and AST) as well as the microscopic observations and microscopic scoring supported the hepatoprotective potential of MEMM.
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