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Microscopic samples were removed for SEM EDS analysis.
Electron microscopic samples were prepared by solidifying a droplet of solution in a refrigerant at very high quenching speed, then replicating it with electron-beam-gunned platinum and carbon.
Microscopic samples were slowly defrosted on ice.
For quantitative assessment, microscopic samples were harvested at 72 h p.i. and individual infection sites were assigned to one of these categories.
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To achieve this, micrograph pairs of the microscopic samples are acquired by utilizing an SEM equipped with motor controlled specimen stage capable of precise translational, rotational movements and tilting of the specimen stage.
After microscopic analysis, samples were individually lysed in 12.5 µl of Sigma Red-extract lysis buffer.
For the microscopic study coal samples were crushed to 18 mesh size (<1 mm size particles).
After microscopic evaluation, OL samples were classified using the binary system [ 27].
After we made microscopic observations, skin samples were processed as described elsewhere (10 ).
For light microscopic analysis, the samples were fixed in 2.5% glutaraldehyde solution, and then dehydrated through a graded acetone series.
For electron microscopic examination, BAT samples were placed in ice-cold fixative buffer (3% gluteraldehyde in 0.2 M sodium cacodylate buffer pH 7.3) for 2 h.
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