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Cultures were fixed and stained for microscopic quantification of multinucleated (MNC) TRAP+ cells.
Additionally, osteoclast activity was determined by light microscopic quantification of resorption pit area on the Bone Cell Assay Surface plates.
Microscopic quantification of this effect at 6 hours post induction revealed that the number of vital transgenic cells was reduced by ∼85% compared to the identically induced wild-type parent strain (Figure 2A).
DNA damage response assays are more usually performed using microscopic quantification of DNA damage foci.
Microscopic quantification of mycorrhizal fungi (AMF) colonization showed an initial appearance of AMF structures at day 9, as well.
Microscopic quantification of positive tumor cells for HCA2, HC10, HLA-E, and HLA-G was performed in a blinded manner by two independent observers (C.C.E., A.S. and A.v.V).
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This study was designed to examine the early development of DN in subjects with T1DM, as determined by electron-microscopic quantification of two research renal biopsies performed 5 years apart (4, 28).
Reliable quantification for both the test organisms was achieved at or above a cell density of 103 cells ml−1, a recognized minimum limit for microscopic quantification.
Microscopic quantification was performed by observers, who were blinded for the identity and grouping of the patient.
After five days of spore formation at 28°C, the cells were removed from the plates, resuspended and the sporulation efficiency determined after microscopic quantification.
BKYB and MPL conducted the experiments and the microscopic quantification.
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