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Nasal mucosal brushings were fixed in glutaraldehyde and routine electron microscopic procedures were carried out.
Initially, microscopic procedures were followed to investigate Lactobacilli sp. presence using wet mount preparation with normal saline.
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The microscopic procedure was performed at a constant room temperature of 21 to 23°C.
The specificity of the Fluorescence microscopic immunohistochemical procedures was validated by omitting the primary antibodies or by using nonimmune serum instead of the primary antibodies.
These procedures were performed under microscopic visualization following removal of debris and cleaning of the external auditory canal.
These procedures were repeated in three microscopic fields for each histological section.
All aspects of the analytical procedures were conducted in laminar flow hoods within the Microscopic and Chemical Analysis Research Center at Ohio State University.
An electron microscopic procedure has been developed, using rat liver, for the localization of hormone-sensitive adenyl cyclase.
During the entire in vivo microscopic procedure, intestine was superfused with thermostatically controlled (37°C [98.6°F]) crystalloid solution (Thomaejonin®) in order to avoid drying and exposure to ambient air [ 12].
However, very few biochemical identification procedures are available for dermatophytes; thus, diagnosis of dermatophyte infections is based on the gross and microscopic morphology of the colony.
Diagnosis of these pathogens is difficult by microscopic procedures and molecular methods have been used as an alternative.
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