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Microscopic observation is conducted to aid in the analysis of the damage mechanism.
Microscopic observation is also used to estimate abundance of microbes, and to a limited extent, assigns microbes to taxa [12].
The area of microscopic observation is originally located just behind the large front vessels that feed and drain the chamber network.
The glutathione beads binding assay coupled with microscopic observation is a variant of the low affinity binding assay developed by Patel et al. (2007).
Fluorescence microscopic observation is one of the most reliable tests for determining and quantifying cell viability and cell death through the use of different cell stains.
Crystal violet staining of surface-attached cells by microtiter plate assay is a popular and convenient method for quantitative detection of bacterial biofilm formation; however direct microscopic observation is strongly recommended to confirm the data from staining analysis [ 12].
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After 3 days of growth at 37°C, positive red clones were observed and fluorescence microscopic observation was performed using a filter Nikon B-2A (Nikon Eclipse E4000).
Microscopic observation was performed with Carl Zeiss Primo Star microscope (Carl Zeiss, Oberkochen, Germany).
Microscopic observation was performed with a microscope (Nikon Eclipse TE300, Nikon, Tokyo, Japan).
Microscopic observation was done using a fluorescence microscope over normal spectra (Olympus DP72, Tokyo, Japan) at 40x magnification.
Fluorescence microscopic observation was performed using an Olympus BX50 microscope (Olympus, Tokyo, Japan).
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