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Microscopic observation for GFP expression was conducted with a MZFLIII fluorescent microscope (Leica Microsystems, Bannockburn, IL) using GFP2 filters and a ProgResCF camera (Jenoptik AG, Jena, Germany) to distinguish GFP expression from autofluorescence in pigmented fish.
The numbers of mycobacteria in the samples were determined by limiting dilution in liquid media, with microscopic observation for growth of characteristic groups of bacteria.
As we always did, each cell sample was divided into two fractions after pre-fixation, immunostaining, and post-fixation, one of which was used for confocal microscopic observation for confirming a good staining status, and the other one of which was for NSOM imaging.
Intravital microscopic observation for diameter measurements was performed under × 200 magnification.
We have compared a PCR assay, based on a repetitive 195-bp segment of nuclear DNA, to direct microscopic observation for diagnosis of T. cruzi infection.
In the work presented here, we have compared a PCR assay and direct microscopic observation for diagnosis of T. cruzi infection in T. infestans collected in the field from five localities and four habitats in Chuquisaca, Bolivia.
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Specifically, such damage evolution is supported by experimental microscopic observations for short glass fiber reinforced polyamide-66.
According to the oral mucous membrane irritation test, scores for macroscopic and microscopic observations for both the aged G/Z and unaged G/Z sides were 0, demonstrating no consequent irritation.
Adult fly brains were dissected in phosphate buffered saline (PBS), and fixed in PBS with 4% formaldehyde for 30 minutes for microscopic observation of endogenous GFP (or RFP/YFP).
For microscopic observation of penetration and infectious hyphae expansion on rice tissue, rice cultivar CO-39 was prepared as previously described [58] and inoculated with 100 µl of conidial suspension (1×104 spores per milliliter) on the inner leaf sheath cuticle cells.
The sections, which are 5 6 μm thick, were then prepared using rotary microtome (Leica RM 2125 RTS, Singapore) and stained with hematoxylin and eosin dye for microscopic observation of histopathological changes in the liver.
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