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An off-axis spherical wave generated from a microscopic objective (0.65 NA) is combined with sequentially illuminated five reference waves at the recording plane.
Two-dimensional fission yeast cell images were collected with a FlowCAM imaging flow cytometer (IFC) with peristaltic pump value of 10, 20× microscopic objective, and 26 particle parameters (Fluid Imaging Technologies, Yarmouth, ME).
The incident laser beam was focused onto the specimen surface through a 100× microscopic objective lens, forming a laser spot of approximately 5 μm in diameter, using a capture time of 50 s.
Raman back-scattered light was collected through a microscopic objective and passed through a holographic edge filter into a multimode fibre (50-μm diameter) and into a 300-mm focal length monochromator, which incorporated a 600/mm grating blazed at 500 nm.
The fluorescence collected by a 40× microscopic objective (UPLANAPO 40×, NA = 0.85, Olympus) was reflected by a dichroic beam splitter (FF665-Di01−25 × 36, Semrock Inc ., and then focused into a multimode fiber by a microscope objective (20×, NA = 0.4, Newport).
Galvanometers (GM1 and GM2, Cambridge Technology Inc). are used as beam positioning and steering elements to make the laser beam moving along a dynamic circular trajectory before entering the inverted microscopic objective; thus, the trapped cell will follow the circular trajectory with a demagnified radius around the optical axis continuously.
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The attempt to resolve the irreversibility paradox along microscopic and objective lines not only enhances the unity of physics and science more generally but it also provides a lesson in the dangers of ad hoc reductionist arguments in general, as well as encouraging an intellectual reconciliation between scientific and wider human experiences.
The number of mitotic figures was identified in 10 high-power microscopic fields (objective magnification × 40).
Percentages were assessed by two independent observers, assuming that a high-power microscopic field (objective x40, microscopic magnification: x400) harbored 100 stromal cells (range: 75 150).
Light microscopic sections were examined using a Leica DMRB brightfield microscope (objectives 1.6× to 20×).
These differences are attributed to the inclusion of individuals with intact tooth crowns and use of objective, microscopic methods to identify LEH.
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