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Microscopic monitoring of floc structure, floc size distribution and the rate of coagulation was carried out for Brownian coagulation of PSL particles.
This screen is based on microscopic monitoring of nuclear pore complexes and nucleolar proteins fused with the green fluorescent protein in a collection of approximately 400 individual deletion mutants.
Each virus also encodes the green fluorescent protein (GFP) for the microscopic monitoring of transduction efficiency and ∼95% of the Schwann cells were transduced, as visualized by the presence of the green fluorescence (data not shown and Figure 1).
Expression of the Beclin-1 constructs was confirmed by western blotting, and induction of autophagy was measured by microscopic monitoring of GFP-LC3 dots.
In other systems such assays are based on the microscopic monitoring of fluorescence changes resulting from the degradation of a reporter protein, termed degron, which is fused to GFP.
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The maximal nontoxic concentration (MNCC), defined as the maximal concentration of the extract that did not exert toxic effect detected by microscopic monitoring, was recorded at the end of treatment.
Online microscopic monitoring ensured that only one cell was aspirated during the process of single cell picking.
The maximal non-cytotoxic concentration (MNCC) was estimated as the maximal concentration of the samples that did not exert toxic effects by microscopic monitoring.
Microscopic monitoring(6), toxicity tests(7), visual inspection.
While each technique has its own advantages and disadvantages, Hoffman modulation contrast microscopy (HMCM) can be considered as the single most beneficial microscopic technique for monitoring of biofilm formation.
Such an instrument could allow microscopic imaging and monitoring of retinal development, disease progression, or the efficacy of therapy in single animals over time.
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