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The maximal non-cytotoxic concentration (MNCC) was estimated as the maximal concentration of the samples that did not exert toxic effects by microscopic monitoring.
Microscopic monitoring of floc structure, floc size distribution and the rate of coagulation was carried out for Brownian coagulation of PSL particles.
This screen is based on microscopic monitoring of nuclear pore complexes and nucleolar proteins fused with the green fluorescent protein in a collection of approximately 400 individual deletion mutants.
The procedure was successfully performed both with simultaneous microscopic monitoring via surgical access, and with fluoroscopical/angiographical guidance solely.
Each virus also encodes the green fluorescent protein (GFP) for the microscopic monitoring of transduction efficiency and ∼95% of the Schwann cells were transduced, as visualized by the presence of the green fluorescence (data not shown and Figure 1).
Frame rate was 4.90 Hz. 5 During microscopic monitoring, cells were maintained at 37°C in a heating chamber.
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We used this system to conduct several operations including a SEM monitored micro house construction by assembling and bonding walls and roof, an optical microscope monitored microscopic insemination by inserting a sperm into an ovum, and a fluorescent microscope monitored DNA surgery cutting out an arbitrary gene from a chromosome.
Such organoids may serve as an inexhaustible source of epithelial cells that can be stored in biobanks and allow medium- to high-throughput screening of CFTR activators, correctors and potentiators on the basis of a simple microscopic assay monitoring organoid swelling.
During this period, confluence was monitored by microscopic observations and by monitoring resistance, using the EVOM resistance meter and the Endohm-12 chamber (World Precision Instruments).
While each technique has its own advantages and disadvantages, Hoffman modulation contrast microscopy (HMCM) can be considered as the single most beneficial microscopic technique for monitoring of biofilm formation.
However, further studies need to focus on the robustness of these proxies, applying isolation and microscopic techniques to monitoring these organisms in the face of oil contamination.
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