Most importantly, we obtained similar results in both agar and broth sporulation assays, and using a microscopic method as well as a conventional spore recovery assay after ethanol shock and growth on agar.
After 48 h or 96 h of culture, samples were analyzed for the presence of vegetative cells and spores, by either recovering viable spores and bacteria on agar, or using a microscopic method, as described below.
The fresh plant material was kept refrigerated and sent to Weleda Naturals GmbH, Schwäbisch Gmünd, Germany, where the identity of the plant material was analyzed by botanic specialists using macroscopic and microscopic methods as well as comparison with depositions of specified botanical reference stocks.
In branched systems, however, the hierarchical nature of relaxations limits direct investigation via these microscopic methods as the dynamic processes are prolongated to much longer relaxation times that are no more accessible to usual dynamic scattering methods.
For pentaerythritol a second order kinetic model was obtained based on the information from trends of the initial reaction rate and the kinetic constants derived from statistical regression using the integral method as well as microscopic observations.
Furthermore, this macroscopic method is the first technique that yields diffusivity values which are in good agreement with those determined using microscopic methods, such as Pulse-Field-Gradient NMR.
The phase separation kinetics of thin films cast onto glass slides and of bulk samples was observed by means of various microscopic methods such as optical, laser scanning confocal, scanning electron and transmission electron microscopy.
In addition, each sample was analyzed in quadruplicate by the direct microscopic method (DMSCC) using Pyronin Y-methyl green as a stain.
To distinguish different forms of CNT agglomerates, their bulk density has so far been measured as a macroscopic method; however, the microscopic method has not been reported.
Using different microscopic and spectroscopic methods, as well as various techniques of mechanical characterization such as tensile testing, microhardness indentation and fracture mechanics, phase behavior and the micromechanical deformation mechanisms active in amorphous styrene/butadiene block copolymers were examined with respect to the molecular architecture.
Therefore, AS-OCT may probably identify failing blebs in a later time with respect to microscopic methods of analysis such as LSCM.
