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This model, spatially constructed solely from the microscopic images, was subsequently validated by our mechanical test results.
Further processing of electron or light microscopic images was done with Photoshop (Adobe Systems), and comprised only linear operations for optimizing brightness and contrast, but no selective processing of image detail.
Photography of light microscopic images was performed with a Powershot G10 camera (Canon, Vienna, Austria).
Quantitative off-line analysis of the microscopic images was performed with the computer-assisted image analysis system Cell-D (Olympus).
The wound area on the microscopic images was calculated using CellSense Dimension 1.6 software (Olympus, Tokyo, Japan).
Revelation was performed using DAB (3,3′-diaminobenzidine). Counterstaining for light microscopic images was performed with hematoxylin.
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Immunostained sections of the mice were observed under a Nikon Eclipse E800 microscope, and light microscopic images were acquired with a digital camera (Nikon DXM1200C).
In two other studies, actual microscopic images were investigated, using microscope navigation data instead of eye movements to study visual processing.
At ten days after explanting, all wells were visualised using the Nikon microscope and camera and light microscopic images were taken of migrating cells from the explants.
Fluorescent microscopic images were visualized on an inverted microscope (Axio Imager M1 microscope, Zeiss, Göttingen, Germany).
Microscopic images were acquired using an inverted microscope (IX 73, Olympus) with a charge-coupled device (CCD) camera (DP80, Olympus).
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