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A sample will be considered negative only after 200 microscopic fields have been evaluated.
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For example, due to their low numbers within the tissue, more microscopic fields had to be analysed for B cells than T cells.
Interestingly, D283 cell line showed strong immunoreactivity with a small number of cells even though the microscopic field shown had more cells.
Both microscopic schemes, confocal and wide-field, have their specific advantages and disadvantages.
The fact that in one case, which exhibited the greatest range of Ki-67-positivity (patient 2), 40 (of 100 possible) microscopic fields would need to have been analyzed to find the 90th percentile of KI-67 expression emphasizes the challenge of dealing with heterogeneity and in developing a strategy to sample the most clinically relevant part of a tumor.
Studies on light field have gotten good results in many research areas, such as microscopic imaging [14], light field camera [15], complex scene 3D reconstruction [16], and accurate depth estimation [17].
No treatment control had 13 tubules (SD = 1.0) per microscopic field and M867 alone had 9 tubules (SD = 1.0), suggesting an anti-angiogenic effect of M867 in addition to the radio-sensitization.
Microscopic analyses indicated that the magnetic field had the effect of forcing the CNTs-Fe to stand perpendicular to the substrate, which causes the enhanced field emission properties.
Each individual experiment had triplicate inserts, and 4 microscopic fields were counted per insert.
The pilot measurement has been carried out on 20 microscopic fields.
The status of cytoskeletal protein polymerization was quantitated by randomly choosing 10 microscopic fields at 40x magnification and counting cells in the field as either having cytoskeletal filaments that were intact or disturbed.
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