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FITC-labeled apoptotic cells within the same microscopic field were viewed and photographed by phase contrast (PC) or by fluorescence emitted at 518 nm (FITC filter).
Of this concentrated sample, 1 mL was used in a Sedgwick-Rafter Cell, of which 100 microscopic field were counted for major species according to Wetzel and Likens (2000).
Only terminal villi showing outlines completely within the microscopic field were analyzed.
Thirty outer hair cells in a given microscopic field were evaluated.
Confocal images were acquired at 63 × and on average eight cells per microscopic field were used for the quantification.
Three hundred cells in total were counted, and one hundred of the cells in each microscopic field were scored.
Similar(47)
The number of cells per microscopic field was counted light microscopically.
The number of DAPI positive nuclei of migrated keratinocytes per microscopic field was counted.
The number of vessels per microscopic field was then determined for each treatment group.
The background of each microscopic field was subtracted with the Subtract Background method of the ImageJ [45] software using a rolling ball radius of 300 [46].
After the experiment the background of each microscopic field was subtracted with the Subtract Background method of the ImageJ software using a rolling ball radius of 300.
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