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The number of cells per microscopic field was counted light microscopically.
The number of vessels per microscopic field was then determined for each treatment group.
The background of each microscopic field was subtracted with the Subtract Background method of the ImageJ [45] software using a rolling ball radius of 300 [46].
After the experiment the background of each microscopic field was subtracted with the Subtract Background method of the ImageJ software using a rolling ball radius of 300.
The cellular TPEF emission spectra for each microscopic field was extracted from these images, after conversion to concentration units and manual selection of a region of interest (ROI) containing the cells and rejecting most of the (non-cell) background.
For each sample, 1 microscopic field was quantified.
Similar(45)
Only terminal villi showing outlines completely within the microscopic field were analyzed.
Thirty outer hair cells in a given microscopic field were evaluated.
Confocal images were acquired at 63 × and on average eight cells per microscopic field were used for the quantification.
Three hundred cells in total were counted, and one hundred of the cells in each microscopic field were scored.
To do so, the nuclei of some infected BMDMØ-GFP in the microscopic field were micro-irradiated by near UV laser (405 nm).
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