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The system is installed in the vacuum chamber of a commercial scanning electron microscope (SEM) and controlled in the microscopic field of view.
The stabilizer was based on application of negative pressure to the periphery of the microscopic field of view to create adherence of the microscope probe to the tissue of interest.
Using this system, they formed a 600 μm long micro-car body shell without dies in a scanning electron microscopic field of view.
In some cases, the areas were located in a band-like distribution (mainly granulation tissue) and the areas were not always large enough to cover the whole microscopic field of view.
The scanning of a microscopic field of mucosa by the laser, combined with the selection of photons originating in the same focal plane ('confocal' plane) results in the imaging of an optical section of this zone of interest.
In addition, because both expressing (MGC803 cell) and non-expressing (GES-1 cell) cells can be simultaneously visualized in a given microscopic field of view, the non-expressing cells could serve as a good control [51].The targeted imaging results are shown in Figure 6.
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Ten randomly selected separate non-overlapping microscopic fields of each kidney section were examined under the light microscope at a magnification (×200) and averaged to yield the score of each kidney.
A mega-image is constructed by registering a sequential set of microscopic fields of view, compressed and stored in hard disk using the JPEG2000 standard (J2K).
After examining approximately 55 microscopic fields of endometrium from each tract, a mean glandular surface density value of 10.2 mm2/mm3 (CE 3.1%) was obtained.
Figure 1B shows microscopic fields of HeLa cells fixed at the indicated time after mitotic selection.
The Virtual Slice module creates a high-resolution montage composed of images obtained from multiple microscopic fields of view.
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