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Combination therapy of M867 and radiation (1.3; SD = 0.57) resulted in a dramatic 5-fold reduction in the average number of vessels per microscopic field in comparison to control (6; SD = 1; p<0.002) and a ∼2-fold reduction relative to radiation therapy alone (2.3; SD = 0.57; p<0.007) (Figure 4A).
Each sample was observed at a 200× magnification of microscopic field in 5 randomly selected areas.
Hematuria was considered as more than five red blood cells per high-power microscopic field in two urine samples.
The lung surface was continuously screened in a standardized manner recording the number of single adherent tumor cells per microscopic field in inspiration as described before [ 10].
Statistical analysis showed that a high S100A9 cell count (> = 200) per 200x magnification microscopic field in cancer tissues was predictive of early stage gastric cancer.
The slides were first overviewed to find five hot spot regions (areas with the maximum number of positive cells per microscopic field) in the tissue studied.
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Microscopic fields in each culture were than analyzed with the WTrack software developed by B. Gönci (unpublished).
In each section, 5 randomly selected microscopic fields in the labyrinthine region (magnification ×40) were acquired at 1280×960 resolution, using a color video camera (Evolution™ MP color, Media Cybernetics) connected to a light microscope (Leica DM LB2, Leica Microsystems).
After the slides were scanned at low magnification, the cells with positively stained nuclei were counted in six microscopic fields in each tumor and the Ki67 index was calculated as the ratio of Ki67 positive nuclei per total nuclei.
24 hours after either a 1 hour or a day long incubation period we fixed the cells and counted the GFP expressing plaques or cells within 30-30 randomicroscopicic fields in each dish.
To quantify PPAR-γ expression, cells in five microscopic fields in each sample were scored as clearly immunopositive or negative for PPAR-γ by two independent examiners, and the ratio of positive cells to total cells in each field was calculated.
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