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During injection and microscopic examining, embryos and larvae were anesthetized in egg water with 0.02% (W/V) buffered 3-aminobenzoic acid (Tricaine; Sigma-Aldrich, A-5040).
During injection and microscopic examining, embryos were anesthetized in egg water with 0.02% (w/v) ethyl-3-aminobenzoate methanesulfonate salt (Sigma).
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For each experimental group, we performed fluorescence analysis of [NO]i and [Ca2+]i on at least 20 cells in the same microscopic field, examined with a 20x objective under a fluorescence inverted microscope equipped with a 75 Watt UV-Xenon lamp (Nikon Diaphot, Tokyo, Japan).
To confirm the presence of Ewing sarcoma xenografts, lungs, kidneys, and macroscopically detected bone and soft tissue tumours were examined by light microscopic examination and by immunohistochemistry. Tissues were fixed in 4% buffered formalin, processed and embedded in paraffin.
The xenografts of wild H460 cells and empty shRNA vector cells showed many papillae, whereas NPC2-depleted cells showed markedly reduced or no papillae formation depending on the microscopic fields examined (Figure 4).
Malaria blood films were prepared and stained with giemsa stain and 100 microscopic fields examined to determine parasite count.
All composites were subjected to DSC measurements for the purpose of examining microscopic miscibility.
The overall multiscale framework is especially suited to biological problems due to the flexibility afforded in specifying microscopic constituents, and examining the effects of various constituents upon overall mucus transport at the continuum scale.
There are several small white Mycena species that are similar in appearance to M. adscendens, some of which can be reliably distinguished only by examining microscopic characteristics.
Gyromitra ambigua is very similar in appearance, and although it is usually not possible to discern between the two species without examining microscopic characteristics, G. ambigua is said to have more pronounced purple tints in the stipe.
These scores are calculated by experienced pathologists based on microscopic observations, through examining hematoxylin and eosin (H&E -stained slides of tH&E -stainedz et al., 2007).
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