Intranasally delivered CTG (6 µl) was detected by microscopic detection of CellTracker Green throughout the hippocampus and cerebellum 5 hours after administration (Figure 2).
For example, as early as 1920, Frost developed a method for rapidly detecting microbial growth by microscopic detection of nascent microcolonies [1].
Microscopic detection was performed under Leica DMLB Fluorescence Microscope.
Cells were incubated with 2 μM CMFDA at 37 °C for 30 min, following which fluorescent microscopic detection was performed under Leica DMLB Fluorescence Microscope.
Commonly used methods for diagnosing Onchocerca volvulus infections (microscopic detection of microfilariae in skin snips and nodule palpation) are insensitive.
(a) Microscopic detection of Bb in a cross-section of a leaf mid rip from a Bb-treated leaf at 200-fold magnification.
Microscopic detection of twitching activity was deemed an indicator of the establishment of bacteria-cell interaction.
As early as 1959, Chance and Thorell reported microscopic detection of single autofluorescent yeast cells [13].
In experimental human malaria infection, complement activation was observed prior to microscopic detection of parasitemia [50].
However, this microscopic detection method is exacting and depends on a good staining technique and well supervised technicians.
This is consistent with the fact that the P. vivax parasitaemias were in most cases below the threshold of microscopic detection.
