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Parasitemia was determined by microscopic counting of blood smears or by fluorescence-assisted cell sorting [FACS].
Urethritis was assessed by microscopic counting of polymorphonuclear leukocytes (PMN) per high power field (HPF); patients with counts of ≥5 PMN/HPF were considered positive for urethritis.
In order to validate the estimates of the normal cell fraction based on the SNP data, a random subset of the 60 NSCLC samples were selected for careful and extensive microscopic counting of normal and tumor cells in frozen sections (see METHODS).
Furthermore, our WPF values are based on microscopic counting of intact spores.
Briefly, the method involves microscopic counting of parasites per field in pure blood.
The differential cell proportion was obtained by microscopic counting of 200 cells.
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Results agree with plate counts and microscopic counts of DAPI stained cells.
Results from these studies demonstrated the aquifer contained relatively low biomass (e.g. direct microscopic counts of <107 bacteria/g of sediment) comprised of a physiologically diverse group of microorganisms including iron reducers, acetogens, sulfate reducers, denitrifiers, aerobic and anaerobic heterotrophs.
The average microscopic count of bacteria in unmineralized dental plaque has been calculated to be up to 2.1 × 10/mg wet weight [ 3, 4].
The results obtained by the qPCR assay correlated closely with the microscopic counts of the four M. avium subspecies strains tested (Table 2).
The average total microscopic count of bacteria from the dental plaque has been calculated to be up to 2.1×10/mg wet weight. 2 3 Paster et al 4 estimated that there are 415 species of non-specific bacteria in the subgingival plaque, while pyro-sequencing techniques analysing dental plaque and saliva have shown that even thousands of microbial species may inhabit the oral cavity.
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