During the cell enumeration procedure, it was noted that the cells at day 7 appeared smaller than at day zero.
Cell enumeration and viability were assessed by use of a hemocytometer and Trypan blue exclusion.
Cell enumeration was performed using a Coulter particle counter (Model 3, Beckman).
The resulting suspension was used for parallel measurements of the impedance response (see below) and for cell enumeration.
Cell enumeration was carried out using a hemocytometer, and viable cells identified by trypan blue exclusion.
The cells proliferation and toxicity assay were preformed by direct cell enumeration.
For cell enumeration, cells were analysed using TrueCount tubes (Becton Dickinson, CA, USA).
CD4+ FoxP3+ Treg cell enumeration was carried out by immunofluorescent staining of intracellular FoxP3.
Per national guidelines, all pregnant women that test HIV-positive should have a CD4 cell enumeration.
Proliferation was determined by measuring both [ 3H]thymidine uptake and cell enumeration using a Coulter counter.
