"microscopic cell counting" is correct and usable in written English.
You can use it whenever you are referring to a microscopic analysis of the number of cells in a sample. For example, "The researcher performed a microscopic cell counting analysis of the sample to determine the cell concentration."
An underestimation of the amount of chromosomal DNA by microscopic cell counting can easily occur due to factors such as cell clumping, cell division or cell lysis.
Also, quality of the tissue digest can be checked by simple microscopic cell counting with dye viability exclusion, an assessment which was sufficient to discriminate between our two different biopsy types.
Briefly, cells from overnight cultures were collected by centrifugation and were used to inoculate plain SVO, after 4 h of growth at 77°C, the culture was then subjected to microscopic cell counting (to determine the total number of cells) and was plated with plain SVO (to find out the number of viable cells).
Direct microscopic cell counting and spectrophotometric readings indicated that cell numbers in flight and ground biological replicate cultures differed by less than 2-fold (data not shown).
The reference was estimated by microscopic cell counting and assuming a chromosomal weight calculated from the chromosome length and GC content of approximately 3.0 fg for S. aureus and 5.7 fg for E. coli, respectively.
As shown by the results, the measured amount of DNA in some cases exceeded that of the reference, which was estimated by the 'gold standard' of microscopic cell counting, resulting in percentages above 100%.
Freezer stocks were revived briefly (<10 minutes) in 9 ml TSB, and microscopic cell counts performed with a hemocytometer were used to standardize the initial inoculum concentration to 4.0 × 105 cells/ml in each well, which contained 20 g/L glucose and TSB.
All samples were separated in two subsamples; one was used for direct microscopic cell counts by Axioscope A1 microscope (Carl Zeiss, Oberkochen, Germany).
These data were compared with traditional metrics, such as microscopic cell counts and analytical toxin measurements, and the public health implications are discussed.
The ARG density determined by qPCR assay is highly related to microscopic cell count (r2 = 0.726, p < 0.001), and the limit of detection (LOD) and limit of quantification (LOQ) of the qPCR method were 0.02 pg and 0.2 pg of DNA, respectively.
However, the deletions that are detected appear to be correct, since the estimates of normal cell content are in agreement with those based on microscopic cell count.
