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Low motile sperm populations were taken for microscopic assay to determine the motility promoting activity of MIF-II antibody.
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A microscopic assay was developed to determine the viability of individual conidia internalized by host cells.
We used a polyclonal-FITC-based immunofluorescence microscopic assay (Pab-IFA) on clinical samples to provide a rapid presumptive diagnosis.
This approach gave results comparable with the epifluorescence microscopy procedure of measuring accumulation of fluorescent dye in living mussel gills (Cornwall et al. 1995; Eufemia and Epel 1998; Galgani et al. 1996) but was less susceptible to subjective errors connected with the microscopic assay, such as the selection of a tissue area to be measured.
The results of these studies indicate that the use of calcium-selective microelectrodes provide a feasible alternative assay of osteoclast activity to the conventional electron microscopic assay.
Furthermore, in our work, the same live cells and direct microscopic assays are used to provide simultaneous insight into replisome behavior, S-G2 phases of the cell cycle, and sister cohesion of the ori1, L3, R3, and ter3 genetic loci.
For microscopic assay, images were acquired using a Nikon 80i Fluorescence Microscope after the cells were stained with Nile red.
The present results of PFGE and electron microscopic assay support our hypothesis that the cell death of irradiated Ax-hp16-infected cells was not due to apoptosis, suggesting that a different biochemical pathway or triggering mechanisms is involved in this mode of cell death.
Chloroquine- and pyrimethamine-resistant P. falciparum K1 strain was exposed to phosphorothioate AS ODNs for 48 h and growth was determined by flow cytometric assay or by microscopic assay.
Such organoids may serve as an inexhaustible source of epithelial cells that can be stored in biobanks and allow medium- to high-throughput screening of CFTR activators, correctors and potentiators on the basis of a simple microscopic assay monitoring organoid swelling.
MSK carried out the immunoassay and microscopic assay.
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