Utilizing epi-third-harmonic-generation (THG) and epi-second-harmonic-generation (SHG) signals, the microscopic cellular and subcellular structures of epithelium and lamina propria in human oral mucosa can be obtained without fluorescence signals, with a lateral spatial resolution better than 500 nm even at 280 μm beneath the surface.
Despite all this, the time of recovery is age dependent, presumably reflecting subclinical (even microscopic) tissue, cellular and subcellular damage (Howlett and Rockwood 2013).
The subcellular fate of fluorescently labeled polymers was monitored by confocal microscopy and subcellular fractionation.
The CCDB was formally launched in 2002 as an on-line repository of high-resolution 3D light and electron microscopic reconstructions of cells and subcellular structures.
Our preliminary results suggest a very good agreement between the simulations of the new discrete model and those obtained by the detailed and subcellular microscopic model.
To determine the relationship between light microscopic types of PrPd and subcellular sites of PrPd accumulation, individual patterns were identified by light microscopy in 1-µm-thick immunolabeled sections and then located in serial sections by electron microscopy.
Microscopic approaches allow the resolution of cellular and subcellular details with high numerical aperture objectives.
MOVID also facilitated direct, intuitive exploration of the relationship between the chemical structures of the probes and their microscopic, subcellular staining patterns.
Microscopic MSI allows multiplexed visualization of unlabeled analytes at cellular and subcellular sizes, but it also involves trade-offs: sampled surface area (per pixel) and detected ions decrease quadratically with probe diameter, while pixel count, and therefore data file size and acquisition time, increase quadratically with spatial resolution.
To bridge the existing gap between cellular and subcellular level, it will be necessary to trace back the mechanical properties of cells to a more microscopic cytoskeletal level.
