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The immunohistochemistry method and the microscopic analysis were described in our previous study [12].
The results from the microscopic analysis were validated by Western blot analysis (Fig. 3B).
Fixation procedure and microscopic analysis were performed as described previously.
Emulsion-dipped [H]AZD2995 labelled tissue sections for microscopic analysis were prepared as described previously [ 13].
Histochemical staining and microscopic analysis were carried out according to Blazquez et al.[ 31].
Differentiation experiments for microscopic analysis were conducted with ASCs from 11 different donors.
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Deconvolution microscopic analysis was carried out using DeltaVision Deconvolution Microscope (Applied Precision, INC).
Microscopic analysis was performed with a Leica DMLB microscope equipped with epifluorescence EL6000 system (Leica Microsystems, Solms, Germany).
The pistils were mounted on a glass slide and microscopic analysis was performed using a Leica DM6000 microscope with a magnification of ×200.
Microscopic analysis was conducted on a Nikon E600 polarizing microscope equipped with a Nikon DXM 1200 digital camera and filters for light polarization.
Microscopic analysis was performed using a confocal laser-scanning microscope (Zeiss LSM 710 META, Germany).
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