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Digoxigenin labelled LNA probes were detected using an anti-DIG fluorescein isothiocyanate (FITC) conjugated antibody and the fluorescent signals visualised with a confocal microscope which permitted the spatial localisation of the miRNAs.
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Stuart Lavery, one of the two consultants at the clinic, talks with pride of its slick operation, the "earthquake-proof" table where sperm and eggs meet, and the joystick-controlled operating microscope, which permits single cells to be taken from embryos and tested for genetic ailments.
For this purpose, an experimental scheme was devised which permitted us to observe, through a high-resolution microscope, cross sections of a ring-shaped hydrate film formed over the surface of each discoid drop of HCFC-141b (Cheldl2F) held stationary in a narrow space between two transparent plates filled with flowing, or quiescent, water.
We later replaced this time-consuming process with functional assessment using a fli1 GFP; gata1:RFP double transgenic line which permitted simultaneous examination of vascular form and function under a fluorescent microscope [ 13].
In contrast, the StdD protocol gives rise to a monolayer of neurons, which permits the use of the Cellomics microscope and BioApplication software to robotically count (cytoplasmic) TH+ neurons.
Imaging of living parasites within the intact spleen was achieved by placing the mice on a stage of a Leica TCS-SP5 microscope fitted with an inverted 63x (1.3 NA) glycerol objective, which permits a working distance of 0.28 mm.
In some microscopes the eyepiece is designed as a portion of a zoom lens, which permits continuous variation of the magnification over a limited range without loss of focus.
Simple scope: This microscope, which weighs just 46 grams, has no lenses.
In a corner was an intravital microscope, which can show cells and tissues in a living animal.
On his desk sits the primary tool of his trade, a microscope, which is used to examine microfibers.
In addition, this work introduced his specially designed field microscope, which subsequently became known as the Withering botanical microscope.
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