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According to these data, and after careful comparison of the microscopic preparations and observation with a stereoscopic microscope, we were able to classify the granulomas according to their major recognizable characteristics (Figure 9 and Table 1) as follows: Phase I granulomas were characterized by the presence of an irregular infiltration with no intragranulomatous necrosis.
Under a powerful scanning electron microscope, we were surprised to see that the super-black feathers look like miniature coral reefs, bottle brushes or trees with tightly packed leaves.
Although it was difficult to discriminate between the intracellular and extracellular fluorescence intensity in the presence of high external fluorescein background due to the limited lateral and longitudinal resolutions of the optical microscope, we were able to correctly determine the position of the cell in the fluorescence image with this procedure.
Although this color change is hard to see by the eye under microscope, we were able to detect it by microspectrometry.
Despite this ambiguity, which is most likely caused by the resolution limit of the microscope, we were able to demonstrate the colocalization of the ubiquitin signal with the late endosomal/lysosomal cellular compartment in pSap deficient neurons.
By introducing an electrically tunable lens into the excitation path of a two-photon microscope we were able to realize fast axial focus shifts within 15 ms. The maximum axial scan range was 0.7 mm employing a 40x NA0.8 water immersion objective, plenty for typically required ranges of 0.2 0.3 mm.
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"Why aren't other people under the same microscope we are?" Dolan asked me.
By including phase masks in the optical path of the microscope we are able to address this non-uniform resolution limitation.
By adding a lenslet array and focusing optics into the optical train of a conventional microscope, we are able to simultaneously capture seven sub-aperture images from different perspectives in a single shot, and finish 3D surface reconstruction of the specimen.
Because of the chromatic error correction system of our electron microscope, we are able to obtain sharp high-contrast images on specimens that are 120 nm rather than the more usual 80 nm.
First, with the help of sophisticated instruments, such as telescopes and electron microscopes, we are able to observe more and more entities, which had to be considered unobservable at a previous stage of scientific and technical evolution.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com