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To ensure further that our image interpretations were not biased by potential differences in intensity of the two fluorescent channels of our microscope, we quantified the ratio of nuclear-to-cytoplasmic NS1 staining for both pAb 155 and mAb 1A7 at each timepoint.
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Using a luminescence microscope, we directly quantified photons from emerald luciferase and red luciferase luminescence independently with different band path filters in the infected limb bud (Materials and Methods; supplementary material Appendix S6).
Budding was quantified using a light microscope, while we quantified myosin ring and SPB status with a fluorescent microscope.
Using an Olympus optical microscope with a 40× objective, we quantified the number of myenteric neurons present in 40 microscopic fields in the intermediate regions (60°-120°, 240°-300°) and 40 microscopic fields in the anti-mesocolic regions (120°-240°) of the intestinal circumference, with 0° as the mesocolon insertion [ 31].
To assess the effects of these compounds on the zebrafish survival rate, we used a conventional microscope and quantified embryo survival rate at 1 and 2 dpf (Table 1).
To examine the impact of MLL4 kncokdown on cell viability, we transfected different types of cultured human cells with MLL4 antisense and examined their morphology under microscope and quantified the cell viability using MTT assay.
The FRET signals were directly visualized under the microscope and quantified as FRET concentration (FRETc) with the Olympus FRET analysis program19,20.
Healing was studied by means of permeability tests on cracked specimens and physical closing of the crack was observed by optic microscope and quantified through crack geometrical parameters.
The ZsGreen1 GFP expression was observed under a fluorescence microscope and quantified by flow cytometry.
For all experiments, three non-overlapping images were acquired with an Olympus FluoView FV1000 laser scanning confocal microscope and quantified using ImagePro software.
Injection of Bla into the cytoplasm of the eukaryotic cells leads to hydrolysis of CCF2 changing its fluorescence emission from 520 nm (green) to 447 nm (blue), which could be detected under the fluorescence microscope or quantified in a fluorimeter.
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