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After examination of 1000 GCs stained by DAPI with a fluorescent microscope, we determined the percentages of apoptotic GCs.
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In order to correct for any possible displacement field due to microscope stage drift, we determined the displacements of beads >15 μm away from the cell, where the influence of the cell traction is negligible, and subtracted these from the displacement field at each node before the traction force computation.
Here, ultrastructure of RBCs is studied using a scanning electron microscope, and we determine how fast changes in healthy individuals are noted after exposure to iron and glucose.
Under an Olympus X51 fluorescent microscope using a triple-filter we determined the percentage of O4-positive cells in relation to >100 DAPI-stained nuclei in randomly selected eye fields for each experimental condition.
Although it was difficult to discriminate between the intracellular and extracellular fluorescence intensity in the presence of high external fluorescein background due to the limited lateral and longitudinal resolutions of the optical microscope, we were able to correctly determine the position of the cell in the fluorescence image with this procedure.
After cyclic aging (80 30 °C cycles for 6 h) of treated Whatman no. 1 paper, no significant changes visible under the microscope could be determined (SEM/light microscope).
The resolution and the light-collecting capability of the microscope are determined by the numerical aperture (N.A). of the objective.
Having examined Ms. Politkovskaya's life "under the microscope," investigators determined that the killer was motivated by her provocative reporting, Mr. Garibyan said.
The limit of resolution of an optical microscope is determined by physical limitation due to the wavelength of light which is around half a micrometer.
The PSF of the microscope was determined using 100 nm fluorescent latex beads (Molecular Probes - Invitrogen, NZ) in the mounting medium.
Spatial resolution of this microscope is determined by the electric field distribution of LSP, and it depends on the polarization of the excitation light.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com