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Under the light microscope, we counted both the total number of neurospheres and the number of large neurospheres over 100 µm in diameter.
Using a 100-point grid of known area (62,500 µm2 at × 400 magnification) attached to the ocular of the microscope, we counted the number of points hitting alveolar tissue and the number of PMN and positive cells in each field.
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We counted conidia under the microscope, with a haemocytometer, to determine inoculum density.
We examined slides under a microscope and counted a minimum of 200 cells to determine mitotic index.
We examined the gut contents under a dissecting microscope, and counted the number of trophozoites, gamonts, and gametocysts.
Migration of the cells into the wounded area was photographed with an Olympus U-RLF-T microscope and counted.
Protoplast release and cell wall degradation were examined with a light microscope and counted using a hemacytometer.
Cells were photographed under the microscope and counted.
The cells were photographed under a fluorescence microscope and counted.
The number of multinucleated cells in five randomly selected areas under the microscope was counted.
Cells were examined using a light microscope and counted in 5 optical fields (100×).
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CEO of Professional Science Editing for Scientists @ prosciediting.com