In combination with an automatically controlled microscope we used the migration chip for screenings of migrating Panc-1 cells using transmitted light and fluorescence live-cell imaging as demonstrated exemplarily in Videos S1, S2, S3.
Photostimulation was carried out using a dual scan head raster scanning confocal microscope and control software developed by Prairie Systems, and incorporated into a BX51 upright microscope (Olympus America).
The cells were maintained at 37°C using an objective heater (Tempcontrol 37-2, Zeiss) and 'The Box' Microscope Temperature Control System (Life Imaging Systems).
For large overviews of the cross and longitudinal sections, we acquired montages of overlapping high-magnification images in an automated fashion using the microscope control software SerialEM (Mastronarde, 2005).
To obtain FE-SEM image of the synthesized material, the instrument Nova Nano SEM 450, make FEI, and software xT microscope control was used.
HelioScan is designed as a collection of components that can be flexibly assembled into microscope control software tailored to the particular hardware and functionality requirements.
Figure 5 shows a screen image from the SQUID microscope measurement and control software SQUID MagScan.
Recording and microiontophoresis were performed with five-barreled glass micropipettes broken back to 8 12 µm under microscope control.
Microscope control and image capture and analysis were accomplished using the Slidebook™ image acquisition and analysis software package (Intelligent Imaging Innovations, Inc., Denver, CO).
Microscope control, image capture, post-processing, and particle tracking of mitochondria were all accomplished using the Slidebook™ image acquisition and analysis software package (Intelligent Imaging Innovations, Inc., Denver, CO).
