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In the DIC view, this area was characterized by the emission of brilliant light, which was enhanced when the DIC analyzer of the microscope was removed (Figure 7: arrows).
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To this aim distorsions introduced by the microscope are removed by deconvolving z stacks containing IN-ECFP or IN-EGFP and lamin A/C Alexa-680 signals using experimentally determined point spread function (PSF) (see Methods).
Under a microscope, clastic material was removed by steel needles to expose the plant morphology.
Under a dissecting microscope, the perineurium was removed and fine neurofilaments were dissected free from the nerve using fine watchmaker forceps.
Under a dissecting microscope, the adventitia layer was removed by microdissection.
A small incision in the middle of the head-neck region was made, and with the aid of an operating microscope, the SM LN was removed bilaterally.
Under general anesthesia, with the use of the operative microscope, the button battery was removed revealing a medium sized central tympanic membrane perforation.
The surface area of the wound was measured at ×20 magnification under an inverted microscope, then the medium was removed and the cells were further incubated for 4 or 18 h with EBM, 5% FBS and VEGF 50 ng/ml.
Sections were transferred to a microscope slide, tissue freezing medium was removed and tissue was fixed with 4% paraformaldehyde (in PBS).
After washing in PBS, surrounding synovial tissue of the TMJ disc was removed under stereo microscope.
The pericardial tissue was removed under a microscope and the LAD artery was ligated with 6 0 silk suture using a snare occluder [ 21].
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