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The microscope was operated with a SlideBook v.6.0.8 software (Intelligent Imaging Innovations).
The microscope was operated at 200 kV and a nominal magnification of 58,000x.
The microscope was operated in vacuum and samples were mounted vertically under an angle of 62° with respect to the primary X-ray beam.
The microscope was operated in STEM mode at an acceleration voltage of 300 kV, providing a probe diameter of 0.5 nm and a zero-loss peak width of 0.15±0.05 eV.
The microscope was operated in HAADF-STEM mode (Z-contrast).
The microscope was operated at an accelerating voltage of 15 kV.
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The microscope was operating in confocal mode, using a 32× infinity corrected Schwarzschild objective (NA = 0.65) and a matching 32× condenser.
The microscope was operating in Tapping/Lift mode to obtain images of both topography and magnetic force over a region of the samples.
When an acoustic microscope is operated in imaging mode (qualitative mode), the image contrast provides a clear distinction of elastic gradients in the surface structure; nevertheless, the resolution is limited to the microscopic level at most [9 12].
Although most STM-TERS microscope are operated in reflection mode, transmission mode measurements on transparent conductive substrates such as indium tin oxide (ITO) and thin coating (<20 nm) of metal on glass substrates can be realised.
The microscope is operated under high vacuum, using immersion mode with a through the lens detector, at a working distance of 5 mm, an accelerating voltage of 10 kV, and a spot size of 2.0.
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