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These slides were studied under a binocular microscope (Vision V4000).
In order to define the cut-offs between high, low and absent ALDH1 expression, 50 randomly selected microscope vision fields were analyzed and a total of 1.500 cells presenting high, low or no ALDH1 expression (500 cells each) were measured by the ARIOL system.
Tumor resection was carried out using the technique described by Saito et al. 44 Under microscope vision, a small incision within the outer layer of the dura was made, which was stripped away from the inner layer using microforceps and microdissectors.
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Cells were imaged on a Zeiss Apotome Fluorescent Microscope (Axio Vision 4.7.Ink), images were deconvolved using Huygens Essential Software Scientific Volume Imaging B.VV). and compiled in Adobe Photoshop.
Time-Laps microscopy was performed using Olympus IX71 microscope (Delta-Vision - Applied Precision).
In addition to this, offline results obtained with a particle vision microscope system are compared to verify the results obtained inline.
A San Francisco startup called 3Scan Inc. raised $14 million in venture funding to automate tissue analysis for scientists involved in drug discovery with its proprietary robotic microscope and computer vision systems.
Images were collected on a Zeiss Axio Observer microscope with Axio Vision software.
All sections were acquired under a Zeiss Axioplan fluorescence microscope, using Axio Vision 4.5 software at a magnification of 20×.
Live cells were visualized using a Zeiss inverted fluorescence microscope with Axion vision software.
Numbers of fluorescence signals were counted independently by two investigators using an Axio Vision microscope (Carl Zeiss, Oberkochen, Germany).
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