In addition two 42-bit color images were captured with a Zeiss AxioCam HR digital camera on a Zeiss Axioskop 2 microscope utilizing AxioVision 4.4 software (Zeiss) of each muscle on the H&E stained slides.
Micrometre sized specimens, rectangular beams produced via focused ion beam milling, were loaded in situ in a scanning electron microscope utilizing a piezo-electrically controlled cube corner micro-indenter.
Figure 1 shows a schematic diagram and a photograph of our lensfree reflection mode microscope utilizing a Michelson interferometer geometry.
Images were collected with a Nikon C1 confocal microscope using a TE2000 PSF inverted microscope, utilizing 60×/NA (numerical aperture) 1.40 Plan Apo or 20×/NA 0.50 Plan Fluor objectives and 3×confocal zoom.
Single focal plane images of the fluorescent signals were acquired with an ORCA-ER digital camera (Hamamatsu) mounted to a Zeiss Axiovert 200M inverted microscope utilizing a 20×, 40× objective and either a green fluorescent protein (GFP) or rhodamine filter cube.
The microscope utilized three separate beam paths for generating focal spots: two at 491 nm wavelength for excitation and off-switching and one at 405 nm for on-switching of the fluorophores.
The scanning electron microscope utilizes electrons to bombard a surface, but the intensity of either backscattered (deflected through angles greater than 90°) or transmitted electrons is measured rather than the intensity of X rays.
The brains (n = 5) were scanned utilizing the Raman microscope (fixed 90° angle of illumination).
