The cover slips were carefully removed and mounted in 60% lactic acid for microscopic observation under the Olympus BX51 microscope, using an Olympus DP12 camera, and the DP software.
The Raman spectra were measured by a Renishaw in via Raman microscope using an excitation laser with a wavelength of 532 nm.
Cross-sectional TEM image was recorded on JEOL JEM 2010F field emission electron microscope, using an accelerating voltage of 200 kV.
Transmission electron microscopy (TEM), EDX, high-resolution TEM (HRTEM) images and mapping images were taken on a JEOL 2010 microscope, using an accelerating voltage of 200 kV.
Scanning electron microscopy (SEM) images were taken with a JEOL-5600LV scanning electron microscope, using an accelerating voltage of 20 kV.
The steel wool was investigated by scanning electron microscope using an energy dispersive analysis system before and after the passing of the flame front.
The number, morphology, and distribution of cells on substrate surface were then evaluated on photographs taken under an Olympus I×51 microscope using an Olympus DP 70 digital camera (Olympus America Inc., Center Valley, PA, USA).
Laser microdissection (LM) is a powerful tool for isolating targeted individual cells from heterogeneous tissue viewed under a microscope, using an intense laser beam (Emmert-Buck et al. 1996).
Images were acquired on a Zeiss AxioplanII microscope using an AxioCamMRm controlled by Axiovision.
Samples were analyzed with an Olympus AX70 Microscope using an UV filter.
Microscopy was performed with an Olympus BX-50 fluorescent microscope using an UPlanFl 40x 0.75 NA objective (Olympus, Tokyo, Japan).
