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Immunostained cells and microvessels were counted microscopically (Nikon E400 microscope) using a 5 × 5 reticle grid.
Samples were analysed using a Leica TCS SP8 confocal microscope using a 40x original magnification.
Images were acquired on a spinning-disk microscope using a 63× objective.
The cell layer around the marked areas was then brushed off under the DIC microscope using a fine needle.
The images were taken under an Olympus BX60F-3 microscope using a 20x and a 40x original magnification.
Confocal fluorescence microscopy was performed on a Leica SP5 confocal microscope using a ×63 oil immersion objective.
The PCR products are imaged on a fluorescence microscope using a hydrolysis probe/primer set (TaqMan).
Injections were done under a dissection microscope using a Hamilton syringe and pulled capillary tubes.
Imaging was taken by fluorescence microscope using a Nikon TE2000s inverted microscope with 10x objective.
Acquisition was performed with a Leica DM4000B microscope using a Leica 10× magnification lens.
Stained tissues were imaged under a binocular microscope using a Nikon Coolpix 990 camera.
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