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Scanning transmission electron microscopy (STEM) under a high-angle annular dark field mode (also called Z-contrast imaging) was conducted using a FEI Tecnai (Hillsboro, OR, USA) F20 microscope (under the operation voltage of 200 KV).
Slides were viewed on the Nikon Eclipse E800 microscope under the 40× magnification.
Digital high-resolution microphotographs were taken with the 10× objective of an Olympus BX41 microscope under the same conditions of light and brightness/contrast.
Cells were counted using a phase contrast microscope under the 20X objective.
Protein levels were scored with a light microscope under the supervision of a pathologist.
Images of resulting embryos were acquired with an inverted microscope under the same exposure parameters.
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After 10 15 days in adhesive culture, neurons and beating cardiomyocytes could be observed under the microscope under all conditions.
Cells from 3 different fields were counted under the light microscope under a magnification of ×200.
Humidity was controlled by placing the microscope under a low flow of dry nitrogen gas.
Surgery is performed with the operating microscope under general anaesthesia.
The fixed samples were visualized under an Olympus SZX12 microscope and pictures taken under the microscope with 16 times magnification.
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