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Positive culture were stained according to the Ziehl-Neelsen method and examined under the microscope to select for acid fast bacilli (AFB) positive isolates.
Longitudinal spinal cord sections stained for ChAT were examined under a fluorescence microscope to select sections that contained motoneuron cell bodies at least 500 µm distal to the lesion scar.
Eyes were then dissected under light microscope to select the tyrosinase-positive portions of the eyecups.
For the ZPA, embryos were examined briefly with a dissecting fluorescent microscope to select those carrying the GFP allele.
After deparaffinisation, tissues stained with methylene blue were dissected manually on an inverted microscope to select melanoma lesions.
For live imaging, hosts were screened at 40 hpf on an upright fluorescent microscope to select those where Atoh7 expression has just started.
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Slides were scanned using a ×4 microscope objective to select areas with highest tumor budding.
In particular, adjusting the variable aperture of the microscope it is possible to select an area of interest in the sample from 250 μm × 250 μm down to approximately 20 μm × 20 μm.
A Leica AS LMD microscope (Leica) was then used to select epithelial cells from tumour biopsies and their distant normal counterpart for each patient.
Small pieces of leaves were excised 2 3 days after infiltration and examined in a confocal laser scanning microscope (Zeiss LSM700) using filters to select for the GFP and chlorophyll signal.
Thus, grains with a diameter comparable to that of a human hair, selected under a microscope to be crack-free and of the highest possible quality, have been found to be more concordant than cracked grains.
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