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During that period he devised methods for using the electron microscope to obtain high-resolution images of individual cells.
Examples will be presented to demonstrate the use of a Scanning Electron Microscope to obtain detailed measurements of hypervelocity impact craters and to obtain the shapes of craters in three dimensions by comparing stereo pairs.
Hyperspectral imaging (HSI) was used in conjunction with the dark-field microscope to obtain reflectance spectra from each pixel in the dark-field image.
Microspheres from both soleus and gastrocnemius (ligated and nonligated hindlimbs) were counted under fluorescent microscope to obtain the perfusion ratio from each animal.
Thus we used the optical zoom feature of the microscope to obtain pixel size that was at the corresponding Nyquist frequency.
The same immunostained cerebellar sections were acquired with a CLSM (Leica SP5, Germany) confocal laser-scanning microscope to obtain images of dendritic Golgi cells (63× oil-objective, 1.4 NA; electronic zoom factor 2.5×).
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Once the tissue is expanded, the researchers can use any of several common microscopes to obtain images with a resolution as high as 60 nanometers — much better than the usual 200 to 250-nanometer limit of light microscopes, which are constrained by the wavelength of visible light.
Physicians often use dermatoscopes or microscopes to obtain a magnified image of the mole.
Experimental images were captured by scanning electron microscope (SEM) to obtain the filling shapes and filling ratios.
The biopsy technique applied was performed specifically to collect vitreous samples as close as possible to the macula, under microscope visualization, to obtain a representative vitreal sample in close proximity to neovascular membranes.
Tissue sections mounted on PEN membrane slides (Leica Microsystems, Wetzlar, Germany) were dissected using the LMD6000 laser microdissection microscope (Leica) to obtain samples of normal mucosa and tumour tissue.
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