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Many precautions must be taken in the design and construction of a polarizing microscope to avoid using optical components that introduce undesirable polarization retardation in the beam of light after it has left the object.
Separately, two samples of somatic tissue (terminal tip of the foot and adductor muscle) were dissected under light microscope to avoid contamination with gonads as well.
Marker positive pixels were highlighted by color thresholding in Adobe Photoshop CS5 using the "wand tool", while simultaneously examining the corresponding glass slides under the microscope to avoid the inclusion of regions that did not correspond to cancer cells.
A single 2- μl injection of 10 ng TNF in 0.01 M PBS, pH 7.40, was administered intravitreally into the right eye of an animal under a microscope to avoid lens injury.
A single 2-μl injection of 10 ng of TNF in 0.01 M PBS, pH 7.40, was administered intravitreally into the right eye of an animal under a microscope to avoid lens injury.
A black box was installed around the stage of the microscope to avoid surrounding light coming in, and the temperature inside this box was controlled at 37 °C using a temperature controller.
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The samples were selected from the Gadvan (Barremian) and Garau (Early Cretaceous) formations using gamma ray logs and binocular microscopes to avoid caving and contamination (Bordenave and Huc 1995).
Use forearm supports or place a lab notebook along the sides of the microscope base to avoid resting on the edge of the base.
An emission short-pass filter (cutoff 690 nm) was placed in the microscope scanhead to avoid background irradiance from the excitation source.
Materials and Methods: Twenty-four fresh-frozen cadaveric knees were dissected using magnifying loupes and an operative microscope, being careful to avoid creating artificially separated bundles.
This sets indirect restrictions on the microscope in order to avoid artifacts.
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