Exact(6)
The stage for the inverted confocal microscope supplied with the HPF machine (EMPACT-2 Leica Microsystems) was not adapted to the use of high magnification lenses (63X, 100X).
Images were taken on a Leica DM-RXA microscope supplied with a Sony 3CCD DXC camera.
The images were taken with an Olympus microscope supplied with a digital camera (DP72, Olympus) at 100 × magnification.
Analysis was performed with a Nikon Eclipse 80i microscope supplied with camera and using the NIS-Elements D software.
Slides were viewed using a BX61 Olympus Microscope supplied with DP50 camera and Viewfinder Lite 1.0 Version (Pixera Corporation) image analysis system.
These plant tissues were screened for GFP expression using a Leica MZ Fluo III (optic 0.63 Zeiss) fluorescence microscope supplied with a DC 300F camera (Leica Microsystems, Welzlar, Germany) with plant GFP filter no. 3 from Leica: excitation wavelengths: 470-540 nm (BP), emission wavelengths: 525-550 nm (BP).
Similar(54)
At least twenty random microscopic fields (400X) and 1000 cells per culture were acquired using a Spot RT digital camera attached to a Nikon Eclipse 600 fluorescence microscope (Nikon Inc), supplied with the adequate excitation and emission filters.
Cells were seeded on coverslips at 50% confluency, 24 h prior to the assembly of the 35 mm dish-type magnetic chamber and the addition of 1 ml of fresh media with MG132, which were then placed into the pre-warmed (37°C) microscope chamber supplied with 5% CO2.
Examination of the slides was carried out at a magnification of 400 times in an epifluorescence microscope (Leitz, Wetzlar, Germany) supplied with a 50 W mercury lamp.
The software supplied with FEI microscopes, Inspect3D, produces reconstructions with the tilt axis in the X-Y plane, so they need to be rotated before further use.
To quantify immunofluorescence of GFP-stained brain sections, fluorescent intensity measurements were taken of a fixed area between sections and within images using software supplied with the LSM510 confocal microscope.
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