Light microscope screening of M. senhousia spawned gametes revealed that both spermatozoa and eggs were fully mature and alive (this has been assessed both by visual inspection and by fecundation attempts that invariably produced viable embryos); also no visible contamination from somatic cells was detected.
In one approach the 'counts' are carried out by viewing the labeled sections directly on the electron microscope viewing screen or via a camera display.
Slides were then examined under a light microscope to screen for cysts of Giardia lamblia, Entamoeba hystolitica, Entamoeba coli and Balantidium coli, ova of Ascaris lumbricoides, Trichuris trichiura and Ancylostoma duodenale and larvae of Strongyloides stercolaris.
Seeds of the T line were treated with EMS and M2 seedlings, which represent the first generation when a recessive mutation can be homozygous, were visualized under a fluorescence stereo microscope to screen for GFP-negative or very weak phenotypes.
Embryos were electroporated with DNA, allowed to develop in ovo for an additional 18-20 hours, and viewed under a fluorescent dissecting microscope to screen for GFP expression, indicating successful electroporation and expression.
Monkeys' parasitemias were assessed daily by reading under fluorescence microscope by screening 1.000.000 Acridine Orange stained RBCs and they were immediately treated whenever P. falciparum infected RBCs were ≥5%, or before if the monkey's health condition had deteriorated.
Using mass spectrometers and powerful microscopes, ProVerde screens weed for bacteria and mycotoxins to make sure it's actually safe to smoke.
These issues are currently being explored and a microscope-based screening platform is being developed with automated sample preparation, image acquisition and data analysis [ 16, 17].
The ScanR microscope-based screening platform was used for automated image acquisition and images were analyzed using the Olympus ScanR Analysis software (Olympus, Hamburg, Germany).
Live-cell imaging can be defined as the study of living cells using images that have been acquired from microscopes or screening systems.
