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Local and international surgeons operated using and teaching microsurgical techniques in teams of two, with additional surgeons observing through an operation microscope observer ocular.
DHE fluorescence signals were detected using a fluorescence microscope (Observer D1, Zeiss, Germany).
Heart sections were stained with hematoxylin/eosin (H/E) and images were captured using a light microscope (Observer D1, Zeiss, Germany).
Images were acquired using a spinning disc confocal system (Wave FX; Quorum Technologies, Guelph, Canada) and microscope (Observer Z1 microscope; Carl Zeiss Microimaging, Jena, Germany) with oil-immersion objectives (PlanApo 40x/1.3 or 63x/1.4 Oil objectives; Carl Zeiss Microimaging).
Briefly, the cells were fluorescently labeled with calcein-AM (green) and ethidium homodimer-1 (red) to visualize the live and dead cells, respectively, using a fluorescence microscope (Observer D1, Zeiss).
Fluorescent images were captured using a fluorescence microscope (Observer Z1; Carl Zeiss, Jena, Germany) connected to a CCD camera (AxioCam MRm; Carl Zeiss) using Axio Vision (version 4.7.2.0; Carl Zeiss) software and exported to Photoshop (Adobe).
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After staining, these histological slides were observed with immunofluorescence microscope (Axio Observer; ZEISS).
These stained cancer cells were observed with immunofluorescence microscope (Axio Observer; ZEISS, Yena, Germany).
The frozen sections of the xenograft tumors were prepared and stained by Alizarin Red S for visualizing calcium deposition and were observed with light microscope (Axio Observer; ZEISS).
A volume of 5 µL of the stained bacterial suspension was trapped between a slide and an 18 mm square coverslip and was observed using a fluorescence microscope (Carl Zeiss, Observer D1).
The colonies were dynamically observed with phase contrast microscope (Axio Observer; ZEISS).
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