The SEM analysis was performed on electron microscope, model LEO-1430-USA LEO-1430-USA LEO-1430-USA seconditionsectrons (ES) with beam current ofor0 μA at constant voltage of 20 kV, workimagesstance of 15 mm; AC2 secondaryelectronsd with a thin layES of platinum in sputter Emitech K550-USA.
The scanning electron microscopy (SEM) techniques using a Jeol scanning electron microscope (Model 1455 LEO) for immobilization of ionophore was used.
This was followed by 20 µl of a 1∶500 dilution of Streptavidin-FITC (Southern Biotech) for 30 minutes at RT. Slides were washed again and Prolong Gold Antifade, (Invitrogen, Carlsbad, CA) was added prior to examination by fluorescence microscopy using a Nikon Eclipse microscope, model, TE 2000-S at a magnification of 400×.
The apparatus was mounted on the stage of an inverted microscope (model IX70; Olympus).
The two Gaussian laser beams were overlapped and directed to the back port of an inverted microscope (model Eclipse TE2000-U, TE2000-U,Nikonn upon Thames, U.Kingston
Images were captured using an EMCCD camera (QuantEM 512SC [Photometrics, Tucson, AZ]), driven by Micromanager mounted on the side port of an inverted microscope (model IX-71; Olympus, Center Valley, PA).
TEM analysis was performed using a transmission electron microscope (Model: 1200EX, JEOL Ltd., Japan).The presence of elemental gold was determined using a scanning electron microscope (Model: EDX Zeiss Evo 50, Carl Zeiss AG, Germany).
The surface morphology of the sample under study in the absence and presence of inhibitors was investigating using a Digital Microscope Imaging scanning electron microscope (model SU6600, serial No. HI-2102-0003) acceleratinging voltage of 40.0 kV.
The diameter and thickness of HF membranes were determined using optical microscope Model B600.POL-I S/N: 0016243, Italy.
The surface morphology of the sample was examined using scanning electron microscope (Model VPFESEM Supra 35VP).
