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An Olympus BX61 microscope, interfaced with Soft Imaging System Colorview video camera and AnalySIS software (all Olympus Tokyo, Japan) was used.
Images of labeled sections were acquired using an Olympus Vanox-S AHBS microscope interfaced with a liquid crystal tunable filter based camera (CRI, Woburn MA, USA) from 420 nm to 720 nm at 20 nm steps.
The samples were examined using a Nikon light microscope interfaced with a spot 24-Bit Digital Color Camera.
Confocal laser scanning microscopy was performed using a Leica LCS (Leica Lasertechnik) instrument based on a Leica DM IRBE microscope interfaced with argon and helium/neon lasers emitting at 488 nm, 543 nm and 633 nm.
Briefly, the quantitative analyses were performed using an Olympus BX61 microscope interfaced with a computer and an Olympus DP71 digital camera, and the NewCAST (Computer Assisted Stereological Toolbox) software package (Olympus, Denmark).
FTIR-RM analyses were performed on the cross sections of Calc1, Calc2, Calc7, Calc8 and Calc10 using a Nic-Plan IR microscope interfaced with a Nicolet Magna-IR 550 FTIR spectrophotometer (Nicolet Instrumentations Inc., Madison, WI, USA) operated in reflectance mode, as previously described [ 14].
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Images were documented with a Nikon TE-2000U inverted microscope interfacing with a Coolsnap fast cooled (ES2) digital camera at 10× (Nikon, Plan Fluor DL, NA 0.3) or 20× (Nikon, Plan Fluor ELWD, NA 0.45) objectives.
Observations were performed by two independent investigators with a Leica DMI 6000B fluorescence microscope (Leica Microsystems) equipped with the software Leica Application Suite version 1.8.0 (Leica Microsystems) or with a Leica MZ10F Modular stereo microscope interfaced to a CCD (charge-coupled device) camera and Leica Application Suite (version 1.8.0) software (Leica Microsystems).
The images of brain sections were projected from an Axioskop Zeiss light microscope using a 10× objective (Zeiss, Gottingen, Germany) through an Axiocam Zeiss high resolution digital camera attached to the microscope and interfaced with a computer.
The RoboLase microscope was interfaced with and controlled a Hamamatsu Orca-AG deep-cooled 1,344×1,024 pixel 12-bit digital CCD camera (Hamamatsu Photonics, K.K., Hamamatsu, Japan).
Immunostaining was analyzed using a fluorescence microscope (Nikon) interfaced with a digital charge-coupled device camera and an image analysis system.
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