Optical spectroscopy was performed on a microscope in dark-field (DF) configuration connected to a CCD and using a confocal fiber collection arrangement coupled to a cooled spectrometer.
In addition, corrosion layers were observed in mounted samples using a polarized light microscope BK-POL/BKPOLR BK-POL/BKPOLRtured by Alltion Comodel in dark field, at Faculty of Conservation, Art University of Isfahan.
This coordinate system allowed us to locate individual nanosponges in the SEM and in the dark field microscope in order to correlate morphology and single nanoparticle scattering spectra.
ALP-positive cells can be visualized in dark purple color under the stereoscopic microscope (Olympus SZX10, Olympus, Tokyo, JAPAN) (Fig. 2).
After 16 hs of treatment in dark seedlings were observed under the microscope.
EYFP fluorescence was observed after 16 h incubation in dark using Leica TCS SP5 confocal microscope.
All the coverslips were sealed onto microscope slides using ProLong Gold antifade reagent (Molecular Probes) and kept in dark for 24 h.
After 16 h incubation at 22°C in dark, protoplasts were observed with LSM 510 confocal microscope (Zeiss).
Slides were cover slipped, cured overnight in dark and examined with EVOS digital inverted fluorescence microscope.
Cells were then incubated with 10 μM CM-H2DCFDA for 30 min in dark, washed with PBS and examined under confocal microscope.
