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Slides were observed using an optical microscope, Imager Z1 (Carl Zeiss).
CK19 and/or CD44 expression was analyzed by a computerized fluorescence microscope (Imager 090 135.001, Leica, Germany).
Cells were viewed with a Zeiss Axio fluorescent microscope (Imager A1) (Carl Zeiss Inc., Thornwood, NY) with an Achroplan 20× and 40× objective that had a numerical aperture of 0.50, fitted with filters for FITC and DAPI identification and equipped with a computer-supported imaging system.
Images were captured using a monochrome digital camera (ORCA-EA, Hamamatsu) mounted on an epi-fluorescence microscope (Imager D1, Carl-Zeiss) equipped with narrow bandpass filters specific for Cy5.5, Cy5, Cy3.5, FITC (fluorescein isothiocyanate) and DAPI fluorescence as well as a 200W metal halide light source (Lumen 200, Prior Scientific).
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Then the biofilm samples were imaged using a fluorescence microscope (Axio Imager M1, Carl Zeiss Inc., Berlin, Germany).
TH27 embryos were imaged using an upright microscope (Axiovision Imager 2e, Zeiss, Jena, Germany), modified for long-term timelapse.
Tissues were imaged using a robotic fluorescence microscope (Zeiss Imager Z1), a cooled, monochrome CCD camera (Retiga 4000R, Qimaging), a motorized slide loader and x-y stage (Ludl Electronic Products) and customized NIH-ImageJ software.
The cells were examined under a microscope (Axio Imager M1; Carl Zeiss, Tokyo, Japan), and images were captured using a digital camera (AxioCam MRm; Carl Zeiss).
Cover slips were air dried and images acquired using a fluorescent microscope (Axio Imager, Carl Zeiss AG, Gottingen, Germany) at a magnification of 400x.
Fluorescent images were acquired on an upright microscope (Axio imager; Carl Zeiss) equipped with 100× oil objectives, NA of 1.46, and a camera (ORCA ER; Hamamatsu Photonics).
Fluorescent images were acquired on an upright microscope (Axio imager; Carl Zeiss) equipped with 100× oil objectives, NA of 1.45, a camera (ORCA ER; Hamamatsu Photonics), and a computer loaded with image-processing software (Axiovision).
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