Illumination, image acquisition, and microscope functions were controlled by Metamorph software (Molecular Devices, Sunnyvale, CA).
All electronic microscope functions as well as camera control, shutters, stage, and focus, are controlled using a home-built software.
To take advantage of the computer controlled microscope functions the OPS imaging probe was attached to the shaft of the microscope.
Transmission electron microscopes function like overhead projectors, with a beam of electrons passing through a slice of material then being projected by lenses on a photographic slide or a charge-coupled device.
We will also only describe the scattering of the electron beam while passing through a sample; probe-forming optics and the microscope transfer function mathematics are described in many other works.
On d14 A4573/GFP cells were quantitated under a fluorescent microscope as function of migration distance from the perimeter of the explant.
We propose a method for microscope point spread function computation in which both design and actual acquisition parameters are explicitly introduced in the integrals describing the electromagnetic field in the focal region.
In addition, further work (e.g. transmission electron microscope, pair distribution function) will be used to look for the simultaneous presence of Ni Zn Al in the hydroxide sheet and their reactivity.
Dynamic plane-stress failure has been observed directly in the transmission electron microscope as a function of temperature using a commercially available cooling/straining holder in conjunction with a copper deformation cartridge.
An additional feature of these samples, however, is that beside a relatively large coumarin emission background, distinct bright spots of the size of the microscope point spread function, are detected (Fig. 1A).
